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Bio-Analytical Separation Science Group


Sebastiaan Eeltink


Research Highlights

COLUMN TECHNOLOGY

MICROFLUIDIC CHIPS

NATIVE PROTEIN ANALYSIS

HIGH-RESOLUTION LC-MS

SPATIAL 3D-LC

DEEP PROTEOME PROFILING

Our research focusses on the design, development, and application of novel analytics for high-resolution profiling of biomolecules and biomolecular interactions using ultra-high-pressure liquid chromatography (UHPLC) and multi-dimensional (2D and 3D) liquid-chromatographic workflows with mass-spectrometry (MS) detection.


The focus is directed towards the development of and application of emerging column technologies to establish bioanalytical separations with unprecedented resolving power. We design novel column structures and chemistries for separations and well as catalyzed reactions. In particular, we focus on polymer monolithic materials.


Novel concepts for multi-dimensional 2D-LC and 3D-LC separations are pursued via microfluidic solutions. We utilize state-of-the-art microfabrication techniques to develop thermoplastic chips with the desired microchannel layout, allowing an enormous versatility in our separation methodologies.


UHPLC-MS and LCxLC-MS method development is conducted in support of post-genomic biotechnology research and medical diagnostics. Our focus is directed to the analysis of highly complex biological samples, which constitutes an enormous number of analytes with a large dynamic range in concentration. Additionally, we target the analysis of emerging biotherapeutics.


The Eeltink Lab also manages the VUB proteomics platform which offers high-performance LC-MS proteomics profiling expertise and advise to academic and industrial collaborators.

News

RECENT PUBLICATIONS

  • Ali Amini co-authored a paper on “Comprehensive quantitative analysis of erythrocytes and leukocytes using trace volume of human blood using microfluidic-image cytometry and machine learning” that has been published in Lab on a Chip 2023, 23, 4868-4875.
  • Zhuoheng Zhou published "A protocol for fabrication of polymer monolithic capillary columns and tuning the morphology targeting high-resolution bioanalysis in gradient-elution liquid chromatography" in the Journal of Separation Science 2023, 46, 2300439.

ANNOUNCEMENTS

  • Zhuoheng Zhou is one of the key contributors driving innovation in the field of separation science at the VUB. Zhuoheng was recently featured in an interview with LC-GC as part of their series “Rising Stars of Separation Science”.
  • Dr. Thomas Themelis was recently honored as one of the recipients of the prestigious Solvay Award for his PhD research on spatial three-dimensional chromatography.


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